The Orchid House
Orchid seeds have only minute reserves of nutrients and need a fungus for germination in nature, this is called "symbiotic" germination. The required nutrients are provided by the fungus. Growth of seedlings is rapid. Until 1922, it was the only known method of propagating orchids from seeds.
In 1922, Dr. Lewis Knudson of Cornell University provided us with a simpler method, "asymbiotic germination." Seeds are germinated and seedlings are grown on a sterile, jelly like, culture medium containing sugars and many other nutrients. Germination takes place without the aid of a fungus. Today, Knudson's formula "C" medium is still being used in laboratories all over the world. Since unwanted bacteria and fungi grow even faster on a sugar rich medium than orchid seedlings, all seeds, flasks, medium, and tools used must be sterilized. All the work involved, like sowing, transferring seedlings etc, is performed in a sterile environment, such as a laminar flow hood or a glovebox.
Sowing of orchid seeds in a "mother flask" is done in a sterile environment, like a laminar flow hood. Filtered through a HEPA filter, sterile air enters the cabinet and passes over the work area. Sowing of seeds is a simple procedure. Germination medium is prepared and poured into clean flasks. Flasks with medium are then autoclaved at 120 degrees Celsius and 15 lbs. pressure for 20 minutes if small quantities of medium per flasks; longer for larger quantities. Cooled flasks are moved into the laminar flowhood where sterilized seeds are distributed evenly on top of the medium. Flasks are tightly sealed and may have a filter to allow air and moisture exchange between inside and outside. The filter must be dense enough to keep micro organisms out. The latter would spoil the germination process. Flasks are then placed under fluorescent lights for a 12 hour photoperiod, for most epiphytic species. Some orchid seeds need total darkness to germinate. After two to three months in the "mother flask", seedlings are large enough to be transferred to a richer medium and larger vessel. The transfer process is called replating.
Replating, or moving seedlings from the old vessel to another with fresh medium, is done in a sterile environment. Seedlings are replated when they have reached a stage of growth at which they will greatly benefit from being placed on nutrient richer medium. Seedlings will now grow very rapidly and usually remain in these flasks for approximately one year, or until they are large enough to be planted out to a community pot.
The community pot may hold from fifteen to 30 seedlings. Plants may spend up to one year in the community pot, before being transplanted to individual pots. After several more transplants and lots of tender loving care in years following, the orchids reach the "flowering size" stage, finally! From seed to flowering plant takes from three to seven years, depending on which orchid species seeds were sown.
Never place flasks in direct sunlight, unles you wish to cook the seedlings.The light should be of low intensity, 300 footcandles, gradually increased as seedlings grow bigger. Place them 30 centimeters below two four feet coolwhite 40Watts fluorescent lamps that cover a growing area of two by four feet. Lights must be on 12 hours per day. Remember the 12 hours uninterrupted darkness is equally important for seedling development.
Temperatures in the 24 degrees C (75 degrees F) range are adequate, give or take 2 degrees C. Keep the variation between day and night time temperatures to an absolute minimum.
Flasks that have filtered tops allow air and humidity exchange of the inside with the environment in which they are placed.To prevent the culture medium from drying out, place flasks in an environment where the relative humidity is about 60%. Keep air movement in the flasking area to a minimum. No fans!
Pour some tepid water into the flasks and carefully swirl it to break up the agar. A spatula may be used to gently aid the breakup. Pour water and seedling mix into a bowl half filled with tepid water. Rinse seedling roots and leaves free of agar. Submerge the seedlings for about ten minutes in a weak systemic fungicide solution, like Benomyl, then place them on paper towels to dry.
This article may be copied, provided credit is given to website and author.